Regulatory
Part:BBa_K1104201:Design
Designed by: Ting-Yun Chiang Group: iGEM13_NYMU-Taipei (2013-09-11)
TrxC promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Primers For PCR
| TrxCp_FP | gct tctagag ccaaagcctgcgactatcatacct |
| TrxCp_RP | gga ctgcag cggccgct actagt a aacgacgccggtatgtttctaatatgt |
PCR Time Protocol
| Temperature(°C) | Time(sec) | Number of Cycles |
|---|---|---|
| 94 | 120 | -- |
| 94 | 15 | 35 |
| 53 | 30 | |
| 72 | 20 | |
| 72 | 300 | -- |
Source
References
- D'Autréaux, B., & Toledano, M. B. (October 01, 2007). ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis. Nature Reviews Molecular Cell Biology, 8, 10, 813-824.
- Ritz, D. (January 28, 2000). Thioredoxin 2 Is Involved in the Oxidative Stress Response in Escherichia coli. Journal of Biological Chemistry, 275, 4, 2505-2512.
- [http://www.ecocyc.org/ECOLI/NEW-IMAGE?type=OPERON&object=TU0-3104 Ecocyc].